以杜仲叶片cDNA为模板,采用反转录RCR及RACE技术分离出DXR基因cDNA全长。序列分析结果表明该基因序列全长1 814 bp,共编码478个氨基酸,推导的蛋白质分子量为51.71 kD,理论等电点5.79,命名为EuDXR。推导的EuDXR蛋白质序列具有植物DXR酶的5个典型基序,并预测出26个潜在的功能位点。系统进化树分析表明EuDXR蛋白与玉米、水稻亲缘关系最近,其次为橡胶、拟南芥、烟草。

The transferability of 53 pairs of self-incompatible S-RNase genotype identification primers from Rosaceae to kernel-apricot was analyzed in order to provide theoretical reference for S-RNase genotype identification and cross breeding work of kernel-apricot. The results showed that only 12 out of 53 primers had good transferability in kernel-apricot which could be used in S-RNase genotype identification. There was a higher transferability in kernel-apricot of Prunoideae than Maloideae, but the highest was in Armeniaca.

In order to set up the genetic diversity system of Eucommia ulmoides Oliver based on SSR molecular markers, the establishment of SSR-PCR reaction system and screening out SSR marker primer showing high polymorphism were studied. A L9(34) orthogonal design was performed to optimize the main factors of the SSR-PCR reaction system. The results indicated that the best SSR-PCR reaction system for E.ulmoides was DNA template 1 μL (30~60 ng·μL-1), 2×Taq PCR Master Mix 10 μL, primer 1 μL with the total volume of 25 μL. The PCR reaction system had high stability and repeatability, the pairs of SSR primers with high polymorphism were gotten. The 8 E.ulmoides samples' DNA sequence was amplified with 13 pairs of SSR primers by SSR-PCR technique, 34 alleles were detected, 2.6 alleles were detected from per site on average. Each allele's effective number was 1.751 5, and the h value was 0.379 8, the average I value was 0.643 3. This study is helpful in using SSR molecular marker to analyze genetic diversity and genetic relationship in E. ulmoides.